RESPIRATORY METABOLISM OF AMPHIBIAN GASTRULA EXPLANTS
نویسندگان
چکیده
منابع مشابه
Incorporation of tritiated cytochalasin B into ectodermal explants of the amphibian gastrula.
Ectodermal explants from gastrulae of Triturus alpestris were exposed to tritiated cytochalasin B (5 and 10 mug/ml) for 1/2-2 1/2 h. The flat explants failed to heal into compact spheres and the component cells gradually rounded up. Radioactivity in the fixed and sectioned material was analysed by light and electron microscopic autoradiography. Silver grains were found predominantly over the yo...
متن کاملCalcium and spreading behaviour of amphibian blastula and gastrula cells.
Cell locomotion involves several structural-functional activities: membrane extensibility, microfilament regulation and adhesive interactions. There is evidence for Ca2+ requirement in all of these. Our data may clarify the role of Ca2+ in locomotion and adhesion. Morphologic and spreading responses of isolated blastula--late gastrula Rana pipiens germ layer cells to varying molar concentration...
متن کاملAmphibian calcium metabolism.
Calcium is present in amphibian blood at a concentration similar to that in other vertebrates, about 1-2 mmol l-1. The fraction of free calcium in amphibians is lower than that in other tetrapod vertebrates because about 50% of the plasma Ca2+ is bound to plasma proteins and perhaps other molecules. Plasma [Ca2+] varies seasonally, increasing in spring and summer and decreasing in winter. Chang...
متن کاملCytological and microspectrophotometric analysis of mesodermalized explants of Triturus gastrula ectoderm.
Using isolated presumptive ectoderm of the newt (Triturus pyrrhogaster) embryos as a reactor and extract of rat bone marrow as a mesodermal inductor, changes of cell number and mitotic index of the reactor cells were studied. In early stages of cultivation the increase in cell number in the mesodermalized ectodermal piece was slower than in the non-mesodermalized epidermal piece; but after 24 h...
متن کاملMorphologic aspects of adhesion and spreading behavior of amphibian blastula and gastrula cells By JACQUELINE
By means of SEM we have examined spreading and adhesive behavior of cells isolated from superficial and deep regions of germ layers from blastula to late gastrula in Rana pipiens embryos. Each of the cell populations sampled show adhesive and spreading characteristics distinctive for each region and stage which we interpret as demonstrating the following: (1) From blastula through late gastrula...
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ژورنال
عنوان ژورنال: The Biological Bulletin
سال: 1952
ISSN: 0006-3185,1939-8697
DOI: 10.2307/1538423